MTHFR Supplementation Protocols banner

MTHFR Supplementation Protocols

Nutrient Combinations Designed to Increase Methylation

Once I Get My Protocol, Where Do I Start?

Start on the step that you think best fits you (if you don’t know, start at the beginning). 


Stop at the step when you are feeling your best. WAIT, what step is that?


For most, if you go one step too far, you may experience some negative reactions, i.e. the feeling of too much caffeine, heart palpitations, anxiety (or even aggression), flu-like symptoms (i.e. fatigue, body aches, etc.). If you're experiencing these things, then you've likely gone beyond a good therapeutic dose for you. In that case, go back to the step before that where you were not having the side effects. And check out our side effects details for more details about how to recognize and manage these, if you should encounter any. 


Each step in your protocol could be defined as 1 day or as many as 7 days (depending upon how sensitive you are to supplement changes, and how carefully you want to track your body’s reactions to any changes). I suspect many will comfortably define a step as 2-5 days.

Quick Personal Note from Methyl-Life® Founder, Jamie Hope

"I had my serotonin level tested before starting a methylfolate and active B12 regimen. My result was 85, significantly low (normal range for the test was 125-185).


3 months later after taking 5 mg of L-Methylfolate and 5 mg of active B12 each day, I had my level tested again and it came back at 136 (significantly improved and within normal range).


Not only did I feel so much better (my mood was lifted), but my IBS symptoms began to melt away (much to my surprise). As I continued taking the nutrients over the next 6-9 months many things just got so much better. It really was quite an amazing transformation for me, a slow but steady healing. So don't underestimate the power of bioidentical methylfolate and B12 nutrients for not only mood improvement, but also repairing the gut.

Additional Resources

Note about B12

Consider taking bioidentical B12 forms in conjunction with L-Methylfolate because they work synergistically together in the body on the methylation pathway and can amplify its benefits. Stay AWAY from cyanocobalamin in supplements and fortified foods! Methyl-Life® carries affordable, vegan versions of active B12 in a tasty sublingual tablet (some products even include L-Methylfolate).


Bioidentical Active B12 Forms:

  • Hydroxocobalamin - harder to find, expensive, typically well-tolerated and used for pernicious anemia among other things
  • Methylcobalamin - fairly available and found in some supplements. Note: it's not always well tolerated
  • Adenosylcobalamin - harder to find, expensive, the most well-tolerated and often used in muscle recovery


B12 does NOT absorb well via the gut, so be sure to get a product that allows you to dissolve the B12 under your tongue. Sublingual transport is the best way to get B12 absorption (research has proven it's even better than B12 injections for increasing B12 sufficiency). If B12 blood serum levels test "high", consider Methyl-Life®'s Non-Methylated Multivitamin.


Learn more about the best bioidentical B12 forms to take.

Select the FREE protocol that best fits you

SENSITIVE Protocol - Vitamin or drug sensitive individuals or people who have complex challenges (i.e. Chronic Fatigue, Lyme, Autism, MS, Fibromyalgia, etc.) or those who carry a significant toxic burden or have an MTHFR variant(s).

PREGNANCY / FERTILITY / CHILDREN Protocol - Those with fertility or pregnancy needs (have had miscarriage(s) or birth defects in the past) OR children who have MTHFR variant(s) that display symptoms like ADHD/Focus/Attention but don't have many other issues. Those who DO NOT experience depression.

GENERAL / CARDIOVASCULAR / DIABETES Protocol - Those with MTHFR variant(s), diabetic neuropathy, high homocysteine levels or cardiovascular health problems. DO NOT experience symptoms of depression or low serotonin levels.

MENTAL HEALTH (DEPRESSION) Protocol - People suffering from low mood, low serotonin levels, depression, IBS, mental or brain health challenges (dementia/Alzheimer's/Bipolar/Cerebral Folate Deficiency/etc.). Likely has MTHFR.

Video Poster

Important things to note before supplementing

... and selecting a specific MTHFR Protocol

Review our dosage information to get some insights from various healthcare professionals about methylation (some suggest low dose while others encourage high dose).

Check out the MTHFR Treatment Tips designed for newbies. It provides some key approaches to the order in which you dose certain nutrients.

Since every person has different genetics, symptoms, toxic burden as well as environmental exposures, there is no one protocol that fits all. And likely what fits you best might be a variation of the protocol you select.

Below are simply 4 specific suggestions to help people start experimenting with nutrients & dosage levels. We encourage start LOW & go SLOW to safely move towards your target goals for methylation & symptom relief.

MTHFR Treatment Tips

Methylfolate Ranges for Specific Health Conditions

If you’re targeting a particular symptom or condition, try a dosage level appropriate to that specific need. NOTE: L-Methylfolate crosses the blood-brain barrier.

Pregnancy/Fertility - target dosage is ~1-4 mg methylfolate per day

Diabetes/Cardiovascular Health - target dosage is ~3 mg methylfolate per day

Lowering Homocysteine levels - target dosage is ~3-7.5 mg methylfolate per day (NAC/NACET, TMG and Glutathione can also help with this)

ADD/ADHD/Focus and Attention - target dosage is ~1-5 mg methylfolate per day

Cognitive Health/Dementia/Alzheimer's - target dosage is ~5-6 mg methylfolate per day

Depression/Bipolar/Mental Health challenges - target dosage is ~7.5-15 mg methylfolate per day

Eye Health - target dosage is ~1 mg methylfolate per day

Table of Contents